Journal: The FASEB Journal

Article Title: CSPG4 Mediates Inflammatory, Cell Death, and Senescence Responses in Enteric Glia Exposed to Clostridioides difficile Toxins

doi: 10.1096/fj.202600333R

Figure Lengend Snippet: CSPG4 blockade reduces TcdB‐induced NFκB nuclear translocation and IL‐6 gene expression. (A) Representative photomicrographs of NFκB (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glia cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glia cells ( n = 5, mean ± SEM) with positive nuclear NFκBp65 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies. (C) IL‐6 gene expression (mean ± SEM) by qPCR in enteric glial cells challenged with TcdA or TcdB at 18 h of incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). (B‐C) One‐way ANOVA followed by the Tukey test.

Article Snippet: Slides were mounted using antifade mounting medium containing phalloidin (Vector Labs, H1600) to visualize the actin cytoskeleton.

Techniques: Translocation Assay, Gene Expression, Immunostaining, Staining, Incubation, Control

Journal: The FASEB Journal

Article Title: CSPG4 Mediates Inflammatory, Cell Death, and Senescence Responses in Enteric Glia Exposed to Clostridioides difficile Toxins

doi: 10.1096/fj.202600333R

Figure Lengend Snippet: CSPG4 blockade reduces TcdB‐induced pSTAT3 nuclear translocation in enteric glial cells. (A) Representative photomicrographs of pSTAT3 (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glial cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glial cells ( n = 5, mean ± SEM) with positive nuclear pSTAT3 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). One‐way ANOVA followed by the Tukey test.

Article Snippet: Slides were mounted using antifade mounting medium containing phalloidin (Vector Labs, H1600) to visualize the actin cytoskeleton.

Techniques: Translocation Assay, Immunostaining, Staining, Incubation, Control